Fascination About Pos88 Slot
Fascination About Pos88 Slot
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Soluble DHFR expression degrees in molecules per cell calculated more info from lysate activity assays as explained in resources and techniques.
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Protein mutational landscapes are shaped via the cellular atmosphere, but important factors and their quantitative effects in many cases are unfamiliar. right here we exhibit that Lon, a high quality Handle protease naturally absent in popular E. coli expression strains, drastically reshapes the mutational landscape in the metabolic enzyme dihydrofolate reductase (DHFR). Selection beneath problems that resolve remarkably Lively mutants reveals that 23.
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